KMID : 0880220220600121153
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Journal of Microbiology 2022 Volume.60 No. 12 p.1153 ~ p.1161
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Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence
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Yongchao Guan
Meng Zhang Yingda Wang Zhongzhuo Liu Zelin Zhao Hong Wang Dingjie An Aidong Qian Yuanhuan Kang Wuwen Sun Xiaofeng Shan
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Abstract
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Aeromonas veronii is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (¥ÄascP) by homologous recombination. The results showed that the deletion of the ascP gene significantly down-regulated the expression of associated effector proteins in A. veronii compared to its wild type. The adhesive and invasive abilities of ¥ÄascP to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of ¥ÄascP to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD50 of the mutant strain of crucian carp was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the ¥ÄascP content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-¥á, and IL-1¥â) in crucian carp after the infection of the ¥ÄascP strain were considerably lower in contrast to the wild strain. Hence, the ascP gene is essential for the etiopathogenesis of A. veronii TH0426.
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KEYWORD
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Aeromonas veronii, ascP gene, T3SS, virulence
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